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2 edition of Lead stress and modulation of TCA cycle enzymes in Pseudomonas fluorescens found in the catalog.

Lead stress and modulation of TCA cycle enzymes in Pseudomonas fluorescens

Jake Bezaire

Lead stress and modulation of TCA cycle enzymes in Pseudomonas fluorescens

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Published by Laurentian University in Sudbury, Ont .
Written in English


Edition Notes

Statementby Jake Bezaire.
The Physical Object
Paginationiii, 32 l. :
Number of Pages32
ID Numbers
Open LibraryOL22161128M

Pseudomonas putida strains are prevalent in a variety of pristine and polluted environments. The genome of the solvent‐tolerant P. putida strain DOT‐T1E which thrives in the presence of high concentrations of monoaromatic hydrocarbons, contains a circular Mbp chromosome and a kbp plasmid. Omics information has been used to identify the genes and proteins involved in solvent. Since P. aeruginosa lacks enzymes required for glycolysis, carbon sources must be converted into tricarboxylic acid (TCA) cycle intermediates before being processed to fructosephosphate via gluconeogenesis. GDP-mannuronic acid is polymerized (Alg8, Alg44, AlgX, and AlgK) and modified (AlgI, AlgJ, AlgF, AlgG, and AlgL) before being exported. Abstract: While the crystal structure of α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) from Pseudomonas fluorescens was solved as a dimer, this enzyme Title: Lutcher Brown Distinguished .   Aluminum is considered the most limiting factor for plant productivity in acidic soils, which cover large areas of the world's potential arable lands. The inhibition of root growth is recognized as the primary effect of Al toxicity. To identify genes associated with Al stress and tolerance, transcriptome analyses of four different wheat lines (2 Al-tolerant and 2 Al sensitive) that differ in.


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Lead stress and modulation of TCA cycle enzymes in Pseudomonas fluorescens by Jake Bezaire Download PDF EPUB FB2

Request PDF | Modulation of TCA cycle enzymes and aluminum stress in Pseudomonas fluorescens | Oxalic acid plays a pivotal role in the adaptation of the soil microbe Pseudomonas fluorescens to.

Modulation of TCA cycle enzymes and the production of oxalate triggered by Al-stress. In conclusion, it appears that the TCA cycle has been reconfigured as a consequence of Al stress.

As the adaptation to this environmental toxicant necessitates the production of oxalate, this cellular system utilizes isocitrate as the generator of by:   As key enzymes of the TCA cycle were ineffective, it was important to decipher how Ps.

fluorescens was metabolizing citrate, the sole carbon source under the challenge of Zn. As pyruvate was an important constituent of the soluble CFE, a metabolite that may be liberated from the tricarboxylic acid, the presence of the enzyme CL was by: Adaptation of Pseudomonas fluorescens to Al-Citrate: Involvement of Tricarboxylic Acid and Glyoxylate Cycle Enzymes and the Influence of Phosphate January Current Microbiology 47(6)   The degradation of Aluminum-citrate by Pseudomonas fluorescens necessitated a major restructuring of the various enzymatic activities involved in the TCA and glyoxylate cycles.

While a six-fold increase in fumarase (FUM EC ) activity was observed in cells subjected to Al-citrate compared to control cells, citrate synthase (CS EC ) activity experienced a two-fold increase.

Pseudomonas fluorescens has been shown to survive a medium with millimolar concentrations of Al, Zn, Fe, Ca and Ga (Appanna and St Pierre, ).The metals are predominantly immobilized as an insoluble pellet that had a characteristic brown-gelatinous appearance (Fig.

1A).Most of the toxic metals have previously been shown to be localized in this pellet (Appanna and St Pierre. The first evidence for the intriguing role of the TCA cycle in modulating oxidative tension was obtained when Ga-citrate was incubated with the CFE (cell-free extract) from P.

fluorescens. 13 C-NMR chemical shifts at 32 ppm and ppm attributable to the CH 2 and COO − of succinate were evident (Figure 1A).On the other hand, the diagnostic fingerprints indicative of KG were present in the.

Lead stress and modulation of TCA cycle enzymes in Pseudomonas fluorescens Jean-Pierre Dionne Détoxification du gallium et les enzymes du cycle de l’acide citrique chez Pseudomonas fluorescens Carrie Mackenzie TCA cycle enzymes and aluminum-citrate metabolism in Pseudomonas fluorescens.

Bédard. Modulation of insect-induced oxidative stress responses by microbial fertilizers in Brassica juncea. India) and contained a mixture of PSB such as Pseudomonas fluorescens, Bacillus megaterium and Bacillus sp.

VAM was obtained from the Division of Microbiology, Indian POD is one of the first enzymes affected by stress in plants. Modulation of TCA cycle enzymes and aluminum stress in Pseudomonas fluorescens.

Hamel RD, Appanna VD J Inorg Biochem, 87(), 01 Nov Modulation of the tricarboxylic acid cycle enzymes by aluminum stress and oxalic acid production in Pseudomonas fluorescens.

Inorgan. Biochem. pdf. V.D. Appanna, R.D. Hamel, E. Pankar and S. Puiseux -Dao () Bioaccumulation of yttrium in Pseudomonas fluorescens and the role of the outermembrane component(s). Pseudomonas fluorescens exposed to ROS can increase the generation of KG through the (i) firstly, modulation of the TCA cycle enzymes, ICDH‐NADP and α‐ketoglutarate dehydrogenase (KGDH).

These enzymes have been shown be up‐regulated and down‐regulated, respectively in the presence of numerous carbon sources including citrate, glucose. Lead stress and modulation of TCA cycle enzymes in Pseudomonas fluorescens book tricarboxylic acid (TCA) cycle is an essential metabolic network in all oxidative organisms and provides precursors for anabolic processes and reducing factors (NADH and FADH2) that drive the generation of energy.

Here, we show that this metabolic network is also an integral part of the oxidative defence machinery in living organisms and α-ketoglutarate (KG) is a key participant in the. However, the expression of some genes encoding key enzymes in the TCA cycle was found to be significantly changed, as demonstrated through RT-qPCR analysis.

In particular, Modulation of TCA cycle enzymes and aluminum stress in Pseudomonas fluorescens. Inorg. Biochem. 87, 1–8. doi: /S(01).

Pseudomonas is a widespread bacterial genus embracing a vast number of species. Various genosystematic methods are used to identify Pseudomonas and differentiate these bacteria from species of the same genus and species of other genera. Ability to degrade and produce a whole spectrum of compounds makes these species perspective in industrial applications.

It also makes. Hamel RD, Appanna VD () Modulation of TCA cycle enzymes and aluminum stress in Pseudomonas fluorescens. J Inorg Biochem 87(1–2):1–8. doi: /s(01) Article.

Growth and nutrient consumption in Pseudomonas monas fluorescens was grown under control and stressed conditions to assess cellular growth patterns. – (closed line) corresponds to the cellular yield of control cultures.

- - (broken line) corresponds to the cellular yield of stressed culture. The consumption of citrate and histidine, the primary nutrients, was monitored by. Recently, it was proposed that there is a common mechanism behind the activity of bactericidal antibiotics, involving the production of reactive oxygen species (ROS).

However, the involvement of ROS in antibiotic-mediated killing has become the subject of much debate. In the present review, we provide an overview of the data supporting the ROS hypothesis; we also present data that explain the. Robert D. Hamel, Vasu D.

Appanna, Modulation of TCA cycle enzymes and aluminum stress in Pseudomonas fluorescens, Journal of Inorganic Biochemistry, /S(01), 87,(), ().

Bacteria belonging to the Pseudomonas genus are highly successful colonizers of the plant rhizosphere. The ability of different Pseudomonas species to live either commensal lifestyles or to act as agents of plant-growth promotion or disease is reflected in a large, highly flexible accessory genome.

Nevertheless, adaptation to the plant environment involves a commonality of phenotypic outputs. Although oxidative stress is known to impede the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, the nutritionally-versatile microbe, Pseudomonas fluorescens has been shown to proliferate in the presence of hydrogen peroxide (H2O2) and nitrosative stress.

THE TEXTBOOK MECHANISM OF TCA CYCLE–GLYOXYLATE SHUNT FLUX PARTITIONING IN E. COLI. The glyoxylate shunt was first discovered in Pseudomonas fluorescens KB1.

However, most of what we now know about its enzymology is based on work carried out in E. coli (34, 35, 43).The first dedicated enzyme in the pathway, isocitrate lyase (ICL; encoded by aceA), is a tetramer that.

Recently, phytoremediation assisted by soil bacteria has emerged as a potential tool to clean up the metal-contaminated/polluted environment.

Three plant-growth-promoting bacteria (PGPBs): Rhizobium sullae, Pseudomonas fluorescens, and Pseudomonas sp. were found to tolerate cadmium (Cd) stress. Sulla coronaria inoculated with these PGPBs, and grown under different Cd concentrations (0.

Since the study of Al3+ ion on the enzyme activity by using of electrochemical techniques was rarely found in available literatures, the differential-pulse polarography (DPP) technique was applied to study the effects of Al3+ ion on the glutamate dehydrogenase (GDH) activity in the catalytical reaction of α-KG +NADH+NH4+ ⇔ L-Glu+NAD++H2O by monitoring the DPP reduction current of NAD+.

The precise partitioning of carbon flux between the TCA cycle and the glyoxylate shunt can be achieved by regulating the activity of IDH through changes in its phosphorylation state. Under normal growth conditions, IDH is mostly unphosphorylated and active.

Thus, most of the carbon flux is directed into the more efficient TCA cycle. The GacS histidine kinase is the membrane sensor of the major upstream two-component system of the regulatory Gac/Rsm signal transduction pathway.

This pathway governs the expression of a wide range of genes in pseudomonads and controls bacterial fitness and motility, tolerance to stress, biofilm formation, and virulence or plant protection.

Despite the importance of these roles, the ligands. Chronic Pseudomonas aeruginosa lung infection is the cause of much morbidity and most of the mortality in cystic fibrosis (CF) patients.

The high prevalence of P. aeruginosa infections in CF is related to the microbe's large genome and mechanisms of adaptation to the CF lung environment, the host immune system and antibiotic resistance.

Among a wide range of P. aeruginosa metabolites. In the study of Arshad et al., pea was inoculated with Pseudomonas fluorescens and Pseudomonas putida showing ACC deaminase activity so as to find their potential to mitigate the effects of drought stress on growth, yield, and ripening of pea (Pisum sativum L.), and it was reported that inoculating with Pseudomonas sp.

decreased the imposed. Several lines of evidence suggest that Al has a strong effect on the TCA cycle and glycolytic pathway.

Modulation in activities of several enzymes involved in synthesis and catabolism of citrate has been reported in yeast and plants (Hoffland et al., ; Neumann et al., ; Zatta et al., ; Neumann and Martinoia, ).

Microbial systems are known to elaborate intricate metabolic strategies in an effort to fend the toxic impact of numerous metals. In this study, we show that the exposure of Pseudomonas fluorescens to aluminum (Al) resulted in a metabolic shift aimed at diverting oxaloacetate towards the biogenesis of an aluminophore.

This metabolic alteration was characterized by uncoupling of two. Vanitha SC, Umesha S () Pseudomonas fluorescens mediated systemic resistance in tomato is driven through an elevated synthesis of defense enzymes. Biol Plant – CrossRef Google Scholar Veena GA, Eswara reddy NP, Harshitha M, Prathyusha C () Efficacy of rhizospheric and root endophytic bacteria against Rhizoctonia bataticola and.

Carbon metabolism plays an essential role in bacterial pathogenesis and susceptibility to antibiotics. In Pseudomonas aeruginosa, Crc, Hfq, and a small RNA, CrcZ, are central regulators of carbon metabolism.

By screening mutants of genes involved in carbon metabolism, we found that mutation of the tpiA gene reduces the expression of the type III secretion system (T3SS) and bacterial resistance.

The reduced DEGs encoding TCA cycle enzymes and mitochondrial respiratory complex subunits were broadly conserved, although less evident in animals. mRNAs encoding pyruvate dehydrogenase kinase, which inhibits pyruvate dehydrogenase, thereby limiting pyruvate entry into the TCA cycle, were induced in studies of humans and plants (OMCL In the current study, the administration of pantothenic acid (26 mg/kg body weight) to normal rats during 6 consecutive days had no significant effect on oxidative stress; the activity of the Krebs cycle enzymes; the activity of transaminases, and the level of neurotransmitter amino acids in the brain, which corroborates the safety of.

Pseudomnas putida is a natural producer of medium chain length polyhydroxyalkanoates (mcl-PHA), a polymeric precursor of bioplastics.

A two-fold increase of mcl-PHA production via inactivation of the glucose dehydrogenase gene gcd, limiting the metabolic flux towards side products like gluconate was achieved before. Here, we investigated the overproduction of enzymes catalyzing.

This appears to be due to deficiency in the gluconeogenic enzymes such as Pps, Pck, some TCA cycle enzymes, the two glyoxylate-shunt enzymes, and certain electron transport carriers. Molecular level research on cra gene expression has been made by several researchers using lacZ -transcriptional fusion [– ].

This would explain the downregulation of aconitate hydratase, an enzyme involved in the TCA cycle with an active [Fe4S4]2+ cluster that is highly sensitive to ROS. In this study, several upregulated proteins grouped as metabolic, oxidative, or general stress responsive proteins were identified (Table 4).

Pseudomonas aeruginosa is an opportunistic human pathogen, particularly noted for causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have shown that the gene expression profile of P. aeruginosa appears to converge toward a common metabolic program as the organism adapts to the CF airway environment.

However, we still have only a limited understanding of how. Nanotoxicological studies were performed in vitro using the common soil bacterium Pseudomonas stutzeri to assess the potentially toxic impact of commercial nano-sized zero-valent iron (nZVI) particles, which are currently used for environmental remediation projects.

The phenotypic response of P. stutzeri to nZVI toxicity includes an initial insult to the cell wall, as evidenced by TEM. Although the citric acid cycle and glyoxylate cycle have many enzymes in common, two enzymes are specific to the latter: isocitrate lyase (ICL), which catalyses the cleavage of isocitrate into glyoxylate and succinate, and malate synthase (MLS), which mediates the condensation of glyoxylate and acetyl‐CoA into malate (Kornberg and Krebs.

Plants are constantly exposed to stressful situations due to changing environmental conditions or through their contact with numerous pests and pathogenic microorganisms that are rapidly evolving to evade host defences. To be more efficient in countering such situations, plants make use of their ‘priming memory’.

Priming (see Glossary) has been defined as an induced state whereby a plant.Pseudomonas aeruginosa is one of the main causes of nosocomial infections and is frequently associated with opportunistic infections among hospitalized patients.

Kaempferol O -(2′,6′-di- O-trans - p- coumaroyl)- β -D glucopyranoside (K F) is an antipseudomonal compound isolated from the leaves of the native medicinal plant Melastoma malabathricum.The Csr (carbon storage regulator) or Rsm (repressor of stationary-phase metabolites) system is among the most extensively studied bacterial RNA-based regulatory systems.

Its central component, the RNA binding protein CsrA (RsmA), was uncovered by a transposon mutagenesis screen designed to identify regulators of gene expression in the stationary phase of growth, using glycogen biosynthesis.